Journal: Journal of Advanced Research

Article Title: Nuciferine inhibits pressure overload-induced cardiac remodeling by activating the SENP1-ACSL4-ferroptosis axis

doi: 10.1016/j.jare.2025.08.020

Figure Lengend Snippet: Nuciferine improves pressure overload-induced cardiac dysfunction in vivo and Ang II-induced cardiomyocyte hypertrophy in vitro . (A) Experimental schedule for TAC modelling and drug administration. (B) Statistical analysis of HW/TL and LW/TL ratios (n = 6 per group). (C) Survival rate in mice after TAC. (D) Representative images of M−mode echocardiography in mice. (E-F) Analyses of cardiac function in mice including heart rate, LVEF, LVFS, LVIDD and LVIDS (n = 6 per group). (G) Relative mRNA levels of Nppa, Nppb and Myh7 in left ventricle tissues, normalized to GAPDH (n = 6 per group). (H) Representative images α-actinin immunofluorescence staining in NRCMs (scale bar = 20 μm, n = 6 per group). (I) Quantification of cardiomyocytes cross-sectional area in NRCMs. (n = 6 per group). (J) Relative mRNA levels of Nppa and Nppb in NRCMs, normalized to GAPDH (n = 6 per group). All data are presented as the mean ± SEM. One-way analysis of variance (ANOVA) followed by Tukey post hoc test was conducted. ns not significant, *P < 0.05.

Article Snippet: After 48 h of co-culture in the aforementioned medium, NRCMs were serum-starved in DMEM/F12 medium for 12 h, followed by treatment with angiotensin II (1 μmol/L, MCE, HY-13948) to induce hypertrophy or Erastin (1 μmol/L,MCE, HY-15763) to establish ferroptosis model. To further elucidate the underlying molecular pathways, genetic manipulations were performed in cultured cells using adenovirus-mediated ACSL4 overexpression (Ad-ACSL4 infection) and Lipo6000TM transfection reagent-mediated (C0526, Beyotime, China) SENP1 silencing (siRNA transfection).

Techniques: In Vivo, In Vitro, Immunofluorescence, Staining

Journal: Journal of Advanced Research

Article Title: Nuciferine inhibits pressure overload-induced cardiac remodeling by activating the SENP1-ACSL4-ferroptosis axis

doi: 10.1016/j.jare.2025.08.020

Figure Lengend Snippet: Nuciferine improves pressure overload-induced cardiac dysfunction in vivo and Ang II-induced cardiomyocyte hypertrophy in vitro . (A) Experimental schedule for TAC modelling and drug administration. (B) Statistical analysis of HW/TL and LW/TL ratios (n = 6 per group). (C) Survival rate in mice after TAC. (D) Representative images of M−mode echocardiography in mice. (E-F) Analyses of cardiac function in mice including heart rate, LVEF, LVFS, LVIDD and LVIDS (n = 6 per group). (G) Relative mRNA levels of Nppa, Nppb and Myh7 in left ventricle tissues, normalized to GAPDH (n = 6 per group). (H) Representative images α-actinin immunofluorescence staining in NRCMs (scale bar = 20 μm, n = 6 per group). (I) Quantification of cardiomyocytes cross-sectional area in NRCMs. (n = 6 per group). (J) Relative mRNA levels of Nppa and Nppb in NRCMs, normalized to GAPDH (n = 6 per group). All data are presented as the mean ± SEM. One-way analysis of variance (ANOVA) followed by Tukey post hoc test was conducted. ns not significant, *P < 0.05.

Article Snippet: After 48 h of co-culture in the aforementioned medium, NRCMs were serum-starved in DMEM/F12 medium for 12 h, followed by treatment with angiotensin II (1 μmol/L, MCE, HY-13948) to induce hypertrophy or Erastin (1 μmol/L,MCE, HY-15763) to establish ferroptosis model. To further elucidate the underlying molecular pathways, genetic manipulations were performed in cultured cells using adenovirus-mediated ACSL4 overexpression (Ad-ACSL4 infection) and Lipo6000TM transfection reagent-mediated (C0526, Beyotime, China) SENP1 silencing (siRNA transfection).

Techniques: In Vivo, In Vitro, Immunofluorescence, Staining